Based on surveys of the literature and herbaria, including internet-available herbarium databases (“virtual herbaria”), plus recent collections, the lichen flora of the Piedmont of North Carolina, USA, consists of 338 taxa representing 110 genera in 41 families including three taxa of uncertain position. Broken down by habit, the flora is 32% crustose, 47% foliose, and 21% fruticose. This report presents the first lichen checklist for the North Carolina Piedmont. Twenty-two taxa are reported as new for the state, including the genera Gyalecta, Lichinia and Sarcogyne.